The ban comes one month after Facebook, YouTube and Apple removed Jones and Infowars' accounts from their platforms, raising pressure on Twitter to follow suit.
After weeks of controversy, Twitter officially banned conspiracy theorist Alex Jones and his InfoWars site from its platform.
Unlike other companies, Twitter CEO Jack Dorsey had initially maintained that Jones should be allowed a place on the platform, allegedly overruling his staff on the matter.
The ban occurred a day after Twitter CEO Jack Dorsey testified to lawmakers about his company's practices for moderating content online. That video showed Jones berating CNN journalist Oliver Darcy for some 10 minutes in between two congressional hearings on social media. The aggressive encounter was filmed by Jones on Periscope, which is owned by Twitter.
In August, Twitter said Jones had violated its rules at least seven times, but it stopped short of banning him outright from the platform.
Before confronting CNN's Darcy, Jones was involved in a heated exchange with Republican Senator Marco Rubio outside of the Senate Intelligence Committee hearing.
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At one point he badgered Rubio as the Republican answered reporters' questions in a hallway. Thus far, efforts to hold Jones accountable to the community guidelines of sites he leveraged have resulted in a almost 50 per cent loss of viewership to Infowars.
"Hey, don't touch me again, man", Rubio said to Jones.
Radio host Alex Jones showed up before a Senate hearing on social media to blast the Democratic party, the leading tech companies and others, claiming they are working together to silence conservative voices ahead of the midterm elections.
He continues by saying Darcy 'smiles like a possum that crawled out of the rear end of a dead cow'.
But a week later Twitter joined the other tech companies in muzzling Jones, even if it was only for a week.
"I know you've got to cover them, but you give these guys way too much attention", Rubio told reporters on Capitol Hill.